Fig 1: Cyclic loading increases the expression and release of ANGPTL4 in tendon cell culture.(a) Western blot analysis of ANGPTL4 protein in total protein extracts of tendon cells with no strain (NS), 2, 4 and 6 hours strain. (b) Quantitation of the Western blot bands by using vinculin as the loading control. (c) Western blot analysis of ANGPTL4 protein in concentrated serum free conditioned media of tendon cells with no strain compared with 2 hours cyclic strain (ST) followed by 6 hours rest. (d) Increased concentration of ANGPTL4 protein in the conditioned media of tendon cells after 6 hours continuous strain compared with no strain (Paired T test; mean ± SE; ****, P<0.0001).